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Methods for Precisely Localized Transfer of Cells or DNA into Early Postimplantation Mouse Embryos
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Chapters
- 00:05Title
- 00:45Dissecting E7.5 or E8.5 Postimplantation Embryos for ex vivo Culture
- 02:03Grafting Cultured Cells into E7.5 or E8.5 Mouse Embryos
- 03:42Handmade Electroporation Materials and Apparatus Setup
- 05:19Electroporation of E7.5 or E8.5 Embryos
- 06:44Results: Localized Cell and DNA Transfer into Mouse Embryos
- 08:15Conclusion
We demonstrate a method for grafting cultured cells into defined sites of early mouse embryos to determine their in vivo potential. We also introduce an optimized electroporation method that uses glass capillaries of known diameter, allowing the precise delivery of exogenous DNA into a few cells in the embryos.
