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JoVE Journal
Developmental Biology

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Methods for Precisely Localized Transfer of Cells or DNA into Early Postimplantation Mouse Embryos
 

Methods for Precisely Localized Transfer of Cells or DNA into Early Postimplantation Mouse Embryos

Article DOI: 10.3791/53295
December 25th, 2015

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Summary December 25th, 2015

We demonstrate a method for grafting cultured cells into defined sites of early mouse embryos to determine their in vivo potential. We also introduce an optimized electroporation method that uses glass capillaries of known diameter, allowing the precise delivery of exogenous DNA into a few cells in the embryos.

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