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In Vitro Disassembly of Influenza A Virus Capsids by Gradient Centrifugation
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Immunology and Infection
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JoVE Journal Immunology and Infection
In Vitro Disassembly of Influenza A Virus Capsids by Gradient Centrifugation

In Vitro Disassembly of Influenza A Virus Capsids by Gradient Centrifugation

DOI:
10.3791/53909-v

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07:24 min

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March 27, 2016

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, ,
1Institute of Biochemistry,ETH Zurich, 2Department of Biochemistry,University of Zurich, 3Institute for Molecular Health Sciences,ETH Zurich

Chapters

  • 00:05Title
  • 01:07Preparation of Glycerol Gradients
  • 02:42Ultracentrifugation of IAV
  • 04:18SDS-PAGE of Pellet Fractions and Coomassie Staining
  • 05:29Results: IAV In Vitro Core Disassembly Assay
  • 06:45Conclusion

Summary

Automatic Translation

Automatic Translation

Disassembly of influenza A virus cores during virus entry into host cells is a multistep process. We describe an in vitro method to analyze the early stages of viral uncoating. In this approach, velocity gradient centrifugation is used to biochemically dissect the steps that initiate uncoating under defined conditions.

Tags

Keywords: Influenza A VirusCapsid DisassemblyGradient CentrifugationEndocytic MilieuPH EffectUncoating MechanismEnvelope VirusesDetergent BufferGlycerol GradientProtease Inhibitor

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