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Immunology and Infection
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抑制接枝反应性免疫的调节性巨噬细胞的功能表征
JoVE Journal
Immunology and Infection
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Immunology and Infection
Functional Characterization of Regulatory Macrophages That Inhibit Graft-reactive Immunity
Please note that all translations are automatically generated.
Click here for the English version.
抑制接枝反应性免疫的调节性巨噬细胞的功能表征
DOI:
10.3791/54242-v
•
08:41 min
•
June 07, 2017
•
Jordi Ochando
2
,
Patricia Conde
2
1
Department of Medicine
,
Icahn School of Medicine at Mount Sinai
,
2
Immunología de Trasplantes, Centro Nacional de Microbiología
,
Instituto de Salud Carlos III
Chapters
00:05
Title
00:45
Graft-infiltrating Macrofage Isolation by Fluorescence-activated Cell Sorting (FACS)
03:23
T Cell Isolation by FACS
06:39
Results: Representative
In Vitro
Macrophage Subset Suppressive Capacity Analyses
07:36
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
巨噬细胞是造血系统的塑料细胞,在保护性免疫和体内平衡方面起关键作用。在本报告中,我们描述了优化
的体外
技术,以表型和功能表征在耐受性条件下积累在移植器官中的移植物浸润调节性巨噬细胞。
Tags
Macrophages
Regulatory Macrophages
Graft-reactive Immunity
Allograft
Single-cell Suspension
Flow Cytometry
Cell Sorting
CD11b
CD45
Ly6C
Ly6G
DAPI
Viability
Cell Culture
Article
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