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Intravital floresan mikroskobu (IVFM) kemik iliği nişler hematopoetik hücrelerin Engraftment Dynamics çalışmaya genetik modelleri ile birleştirerek
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Combining Intravital Fluorescent Microscopy (IVFM) with Genetic Models to Study Engraftment Dynamics of Hematopoietic Cells to Bone Marrow Niches
DOI:

11:06 min

March 21, 2017

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Chapters

  • 00:05Title
  • 01:26Donor Lysozyme Promoter (Lys)-EGFP Mouse Bone Marrow Cell Preparation for Bone Marrow Cell Transplantation (BMT)
  • 03:16Lys-GFP BMT and Intravital Imaging Preparation
  • 05:23In Vivo High Resolution Mouse Calvarium Imaging
  • 07:15Quantitative Analysis
  • 08:14Results: Representative Kinetics and Localization of Myeloid Cell Engraftment Following BMT
  • 10:10Conclusion

Summary

Automatic Translation

İntravital Floresans mikroskobu (IVFM) hasta, posta ve kemik iliği (BM) niş içine hematopoetik hücrelerin engraftment çalışmaya genetik hayvan modelleri ile birlikte uygulanır.

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