Genetic and Biochemical Approaches for In Vivo and In Vitro Assessment of Protein Oligomerization: The Ryanodine Receptor Case Study

Paulina J. Stanczyk; F. Anthony Lai; Spyros Zissimopoulos

doi:10.3791/54271

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Approches génétiques et biochimiques pour In Vivo et In vitro Évaluation de Protein oligomérisation: Étude de cas Ryanodine Receptor

Genetic and Biochemical Approaches for <em>In Vivo</em> and <em>In Vitro</em> Assessment of Protein Oligomerization: The Ryanodine Receptor Case Study

JoVE Journal
Biology

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JoVE Journal Biology

Genetic and Biochemical Approaches for In Vivo and In Vitro Assessment of Protein Oligomerization: The Ryanodine Receptor Case Study

Approches génétiques et biochimiques pour In Vivo et In vitro Évaluation de Protein oligomérisation: Étude de cas Ryanodine Receptor

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11,636 Views

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12:43 min

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July 27, 2016

DOI:

10.3791/54271-v

DOI:

10.3791/54271-v

•

12:43 min

July 27, 2016

•

11614 Views

Paulina J. Stanczyk¹, F. Anthony Lai¹, Spyros Zissimopoulos¹

¹Wales Heart Research Institute,Cardiff University School of Medicine, College of Biomedical and Life Sciences

Summary

Oligomerization of the ryanodine receptor, a homo-tetrameric ion channel mediating Ca²⁺ release from intracellular stores, is critical for skeletal and cardiac muscle contraction. Here, we present complementary in vivo and in vitro methods to detect protein self-association and determine homo-oligomer stoichiometry.