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机器人平台的高通量原生质体分离和转型
JoVE Journal
Genetics
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JoVE Journal
Genetics
A Robotic Platform for High-throughput Protoplast Isolation and Transformation
Please note that all translations are automatically generated.
Click here for the English version.
机器人平台的高通量原生质体分离和转型
DOI:
10.3791/54300-v
•
10:12 min
•
September 27, 2016
•
Elizabeth M. Dlugosz
,
Scott C. Lenaghan
3
,
C. Neal Stewart, Jr.
1
Department of Plant Sciences
,
University of Tennessee, Knoxville
,
2
Center for Renewable Carbon
,
University of Tennessee, Knoxville
,
3
Department of Mechanical, Aerospace, and Biomedical Engineering
,
University of Tennessee, Knoxville
Chapters
00:05
Title
00:40
Establishment of Suspension Cell Cultures
01:40
Set up of Automated Protocols for Protoplast Isolation and Transformation
05:16
Automated Protocols
06:37
Microscopic Analysis of Transformation
07:42
Results: Rapid, Automated, High-throughput Screening of Plant Protoplasts
09:40
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
高通量,自动化,烟草原生质体的生产和改造的方法进行描述。该机器人系统可以在模型BY-2系统应该是翻译到非模式作物大规模并行基因表达和发现。
Tags
Protoplast Isolation
High-throughput Screening
Plant Biotechnology
Automated Protoplast Production
Protoplast Transformation
BY-2 Suspension Culture
Microplate Mover
Liquid Handler
Multi-mode Plate Reader
Multi-mode Dispenser
Plate Heater
Plate Chiller
Robotics System
Plate Mover Software
Labware
Container Types
Start And End Positions
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