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En robot plattform for High-throughput Protoplast Isolering og Transformation
JoVE Journal
Genetics
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JoVE Journal Genetics
A Robotic Platform for High-throughput Protoplast Isolation and Transformation
DOI:

10:12 min

September 27, 2016

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Chapters

  • 00:05Title
  • 00:40Establishment of Suspension Cell Cultures
  • 01:40Set up of Automated Protocols for Protoplast Isolation and Transformation
  • 05:16Automated Protocols
  • 06:37Microscopic Analysis of Transformation
  • 07:42Results: Rapid, Automated, High-throughput Screening of Plant Protoplasts
  • 09:40Conclusion

Summary

Automatic Translation

En høy gjennomstrømming, automatisert, tobakk protoplast produksjon og transformasjon metodikk er beskrevet. Den robotsystem gjør massivt parallell genekspresjon og funn i modellen BY-2 system som skal være oversettes til ikke-modellen avlinger.

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