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经典霍奇金淋巴瘤的芦苇细胞的流分选和外显子测序
JoVE Journal
Genetics
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JoVE Journal Genetics
Flow-sorting and Exome Sequencing of the Reed-Sternberg Cells of Classical Hodgkin Lymphoma
DOI:

08:53 min

June 10, 2017

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Chapters

  • 00:05Title
  • 01:19Cell Suspension Preparation for Sorting
  • 03:00Compensation Control Setup
  • 04:53Hodgkin Reed-Sternberg-, B-, and T-cell Isolation
  • 06:07Results: Representative DNA Library and Copy Number Gains and Losses Analyses
  • 08:02Conclusion

Summary

Automatic Translation

在这里,我们描述了一种组合流式细胞分选和低投入的下一代图书馆建设协议,旨在从古典霍奇金淋巴瘤(CHL)的霍奇金Reed-Sternberg(HRS)细胞产生高质量的全外显子数据。

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