JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Genetics
/
经典霍奇金淋巴瘤的芦苇细胞的流分选和外显子测序
JoVE Journal
Genetics
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Genetics
Flow-sorting and Exome Sequencing of the Reed-Sternberg Cells of Classical Hodgkin Lymphoma
Please note that all translations are automatically generated.
Click here for the English version.
经典霍奇金淋巴瘤的芦苇细胞的流分选和外显子测序
DOI:
10.3791/54399-v
•
08:53 min
•
June 10, 2017
•
Jonathan B. Reichel
,
Jason McCormick
,
Jonathan R. Fromm
,
Olivier Elemento
,
Ethel Cesarman
,
Mikhail Roshal
1
Innovation Laboratory, Center for Molecular Oncology
,
Memorial Sloan Kettering Cancer Center
,
2
Flow-Sorting Core Facility
,
Weill Cornell Medical College
,
3
Department of Laboratory Medicine
,
University of Washington
,
4
Department of Physiology and Biophysics, Institute for Computational Biomedicine
,
Weill Cornell Medical College
,
5
Department of Pathology and Laboratory Medicine
,
Weill Cornell Medical College
,
6
Department of Laboratory Medicine
,
Memorial Sloan Kettering Cancer Center
Chapters
00:05
Title
01:19
Cell Suspension Preparation for Sorting
03:00
Compensation Control Setup
04:53
Hodgkin Reed-Sternberg-, B-, and T-cell Isolation
06:07
Results: Representative DNA Library and Copy Number Gains and Losses Analyses
08:02
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
在这里,我们描述了一种组合流式细胞分选和低投入的下一代图书馆建设协议,旨在从古典霍奇金淋巴瘤(CHL)的霍奇金Reed-Sternberg(HRS)细胞产生高质量的全外显子数据。
Tags
Flow-sorting
Exome Sequencing
Reed-Sternberg Cells
Classical Hodgkin Lymphoma
CHL
HRS Cells
Next-generation Sequencing
Tumor-derived DNA
Diagnosis
Classification
Prognosis
Therapy
Flow Cytometry
Antibody Cocktail
Thawing Medium
Sorting Medium
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
单细胞测序拷贝数改变的检测
的转录分析<em>ç</em>。<em>线虫</em> RNA测序数据通过炫套房的银河项目
G2-seq: 一种基于高通量测序技术的基因组晚期复制区域识别方法
植物标本标本的鲁棒 DNA 分离与高通量测序库建设
用于下一代测序的近全长 HIV-1 Proviruses 的扩增
利用下一代序列染色体构象捕获 (4 c 序列) 的高通量识别基因调控数列
人骨髓间充质干细胞细胞外微 rna 的分离、测序和分析
一种在裂变酵母中的深度固序辅助自发抑制屏
用于抗倍体植入前基因测试的半导体测序
纳米测序全血 mRNA 测序
Read Article