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JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
En ikke-invasiv og Teknisk Ikke-intensive Metode til induktion og fænotype for Eksperimentel bakteriel lungebetændelse i mus
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En ikke-invasiv og Teknisk Ikke-intensive Metode til induktion og fænotype for Eksperimentel bakteriel lungebetændelse i mus

Article DOI: 10.3791/54508
September 28th, 2016 •
1, 1
1Immunity, Inflammation and Disease Laboratory, National Institute of Environmental Health Sciences (NIEHS), National Institutes of Health

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September 28th, 2016

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Adskillige fremgangsmåder er blevet beskrevet i litteraturen til modellering bakteriel lungebetændelse i mus. Heri beskriver vi en ikke-invasiv, billig, hurtig metode til induktion lungebetændelse via aspiration (dvs. inhalation) af et bakterielt podestof pipetteret ind i oropharynx. Downstream metoder til vurdering af pulmonal medfødte immunforsvar er også detaljeret.

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