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在拟南芥原生质体转染昼夜的表型有荧光时钟记者的快速分析
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Rapid Analysis of Circadian Phenotypes in Arabidopsis Protoplasts Transfected with a Luminescent Clock Reporter
Please note that all translations are automatically generated.
Click here for the English version.
在拟南芥原生质体转染昼夜的表型有荧光时钟记者的快速分析
DOI:
10.3791/54586-v
•
07:42 min
•
September 17, 2016
•
Louise L. Hansen
,
Gerben van Ooijen
1
Institute for Molecular Plant Sciences
,
University of Edinburgh
Chapters
00:05
Title
00:39
Protoplast Isolation
03:16
Protoplast Transfection
05:03
Imaging and Data Analysis
05:48
Results: Detection of Altered Circadian Rhythms in
Arabidopsis
Protoplasts
07:02
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
生物钟的调节有关拟南芥转录组的三分之一,但基因的反馈到报时的比例尚不清楚。在这里,我们可视化的方法来使用一昼夜记者在原生质体瞬时表达的荧光成像拟南芥突变体的任何行快速评估昼夜的表型。
Tags
Arabidopsis
Protoplasts
Circadian
Luminescent
Reporter
Circadian Period
Circadian Biology
Gene Feedback
Transgenic
Enzyme Solution
Epidermis
Mesophyll Cells
W5 Solution
Hemocytometer
MMg Solution
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