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Hurtig Analyse af døgnrytmen fænotyper i Arabidopsis Protoplaster transficeret med et Selvlysende Clock Reporter
JoVE Journal
Biology
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JoVE Journal Biology
Rapid Analysis of Circadian Phenotypes in Arabidopsis Protoplasts Transfected with a Luminescent Clock Reporter

Hurtig Analyse af døgnrytmen fænotyper i Arabidopsis Protoplaster transficeret med et Selvlysende Clock Reporter

DOI:
10.3791/54586-v

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07:42 min

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September 17, 2016

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,
1Institute for Molecular Plant Sciences,University of Edinburgh

Chapters

  • 00:05Title
  • 00:39Protoplast Isolation
  • 03:16Protoplast Transfection
  • 05:03Imaging and Data Analysis
  • 05:48Results: Detection of Altered Circadian Rhythms in Arabidopsis Protoplasts
  • 07:02Conclusion

Summary

Automatic Translation

Automatic Translation

Circadian ur regulerer omkring en tredjedel af Arabidopsis transkriptomet, men andelen af ​​gener, der føres tilbage til tidtagning fortsat ukendt. Her visualisere vi en metode til hurtigt at vurdere cirkadiske fænotyper i nogen mutant linje af Arabidopsis hjælp luminescerende billeddannelse af en circadian reporter transient udtrykt i protoplaster.

Tags

ArabidopsisProtoplastsCircadianLuminescentReporterCircadian PeriodCircadian BiologyGene FeedbackTransgenicEnzyme SolutionEpidermisMesophyll CellsW5 SolutionHemocytometerMMg Solution

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