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JoVE Journal Immunology and Infection
Fluorescence-tri cellulaire activé pour la purification de cellules dendritiques plasmacytoïdes de la moelle osseuse de souris
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Fluorescence-tri cellulaire activé pour la purification de cellules dendritiques plasmacytoïdes de la moelle osseuse de souris

Article DOI: 10.3791/54641-v • 08:01 min • November 4th, 2016
November 4th, 2016 •
1, 2, 1
1Department of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary Medicine, Virginia Polytechnic Institute and State University, 2Flow Cytometry Laboratory, Virginia-Maryland College of Veterinary Medicine, Virginia Polytechnic Institute and State University

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Nous rapportons un protocole utilisant les cellules activées par fluorescence de tri pour isoler les cellules dendritiques plasmacytoïdes (pDC) avec une grande pureté à partir de la moelle osseuse de souris lupus sujettes à des études fonctionnelles de pDC.

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Immunologie numéro 117 FACS souris la moelle osseuse les cellules dendritiques plasmacytoïdes (pDC) IFNa le lupus
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