Journal
/
Immunology and Infection
/
荧光激活细胞分选从小鼠骨髓浆树突状细胞的分离纯化
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Fluorescence-activated Cell Sorting for Purification of Plasmacytoid Dendritic Cells from the Mouse Bone Marrow
Please note that all translations are automatically generated. Click here for the English version.

荧光激活细胞分选从小鼠骨髓浆树突状细胞的分离纯化

DOI:
10.3791/54641-v

•

08:01 min

•

November 04, 2016

•

, ,
1Department of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary Medicine,Virginia Polytechnic Institute and State University, 2Flow Cytometry Laboratory, Virginia-Maryland College of Veterinary Medicine,Virginia Polytechnic Institute and State University

Chapters

  • 00:05Title
  • 00:49Bone Marrow Cell Isolation
  • 02:30Cell Surface Staining for Fluorescence-Activated Cell Sorting (FACS)
  • 04:09Plasmacytoid Cell FACS Purification
  • 05:48Results: IFNalpha Production by FACS-Sorted pDC
  • 07:10Conclusion

Summary

Automatic Translation

Automatic Translation

我们使用荧光激活细胞分选来分离浆细胞样树突细胞（PDC）与来自狼疮易感小鼠的骨髓供的pDC的功能研究的高纯度报告的协议。

Tags

Fluorescence-activated Cell SortingPlasmacytoid Dendritic CellsBone MarrowLupusImmune CellInterferon AlphaDensity GradientCell Surface StainingFACS

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

特定细胞群的净化荧光激活细胞分选（FACS）

小鼠骨髓来源的树突状细胞与跟踪研究的Qdot纳米晶的生成及标签

区分功能基因表达的作用，通过骨髓移植免疫和非免疫细胞在小鼠结肠炎

功能研究和过继转移实验小鼠骨髓嗜中性粒细胞的分离，纯化及贴标

评估小鼠浆细胞树突状细胞在淋巴集结的开发利用造血祖细胞的过继转移

在体外产生的常见淋巴祖使用AC-6馈线系统的小鼠浆细胞样树突细胞

代和GM-CSF的鉴定源性肺泡状巨噬细胞和树突状细胞从小鼠骨髓

利用新的细胞分选策略, 从小鼠骨髓中生成大量的髓样祖细胞和树突状体细胞前体

小鼠骨髓基质树突状细胞诱导 CD4 T 细胞活化、增殖和 Th1 分化的体外模型研究

使用胶原酶E从毛里结肠分离拉米纳普里亚单核细胞

Read Article