Journal
/
Biology
/
Visualizing the Early Stages of Phagocytosis
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Visualizing the Early Stages of Phagocytosis

Visualizing the Early Stages of Phagocytosis

DOI:
10.3791/54646-v

•

08:04 min

•

February 03, 2017

•

, ,
1Department of Molecular Microbiology & Immunology,Oregon Health & Science University, 2Ragon Institute of MGH, MIT and Harvard

Chapters

  • 00:05Title
  • 00:38Phagocytosis Binding Assay
  • 03:58Phagocytosis Uptake Assay
  • 04:50Live Imaging of Phagocytosis
  • 06:03Results: Sphingolipids Knockout Cells Show Reduced Phagocytosis
  • 07:03Conclusion

Summary

Automatic Translation

Automatic Translation

Here we describe a microscope-based technique to visualize and quantify the early cascades of events during phagocytosis of pathogens such as the fungi Candida albicans and particulates that are larger than 0.5 µm including zymosan and IgG-coated beads.

Tags

Keywords: PhagocytosisConfocal MicroscopePathogenHost CellVirusFluorescently Conjugated ZymosanDC 2.4 CellsEndocytosisCytosisCell Culture MediumMultiplicity Of InfectionCentrifugationParaformaldehydeAmmonium Chloride

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/54646?article_type=v

Layers of Symbiosis – Visualizing the Termite Hindgut Microbial Community
check_url/54646?article_type=v

Visualizing the Live Drosophila Glial-neuromuscular Junction with Fluorescent Dyes
check_url/54646?article_type=v

Visualizing the Beating Heart in Drosophila
check_url/54646?article_type=v

Tracking Morphogenetic Tissue Deformations in the Early Chick Embryo
check_url/54646?article_type=v

Live Cell Imaging of Early Autophagy Events: Omegasomes and Beyond
check_url/54646?article_type=v

Dissection and Downstream Analysis of Zebra Finch Embryos at Early Stages of Development
check_url/54646?article_type=v

Xenopus laevis as a Model to Identify Translation Impairment
check_url/54646?article_type=v

Isolation and Culture of Human Mature Adipocytes Using Membrane Mature Adipocyte Aggregate Cultures (MAAC)
check_url/54646?article_type=v

A Pipeline using Bilateral In Utero Electroporation to Interrogate Genetic Influences on Rodent Behavior
check_url/54646?article_type=v

Dissecting, Fixing, and Visualizing the Drosophila Pupal Notum

Read Article