Visualizing the Early Stages of Phagocytosis

Ali Rashidfarrokhi; Veronica Richina; Fikadu G. Tafesse

doi:10.3791/54646

Journal

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Biology

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Visualizing the Early Stages of Phagocytosis

JoVE Journal
Biology

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JoVE Journal Biology

Visualizing the Early Stages of Phagocytosis

16,635 Views

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08:04 min

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February 03, 2017

DOI:

10.3791/54646-v

DOI:

10.3791/54646-v

•

08:04 min

February 03, 2017

•

16587 Views

Ali Rashidfarrokhi*², Veronica Richina*², Fikadu G. Tafesse^1,2

¹Department of Molecular Microbiology & Immunology,Oregon Health & Science University ²Ragon Institute of MGH, MIT and Harvard

Summary

Here we describe a microscope-based technique to visualize and quantify the early cascades of events during phagocytosis of pathogens such as the fungi Candida albicans and particulates that are larger than 0.5 µm including zymosan and IgG-coated beads.