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Visualisering af trækul Agar Resazurin Assay til Semi-kvantitativ, Medium-throughput Tælling af mykobakterier
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Visualization of the Charcoal Agar Resazurin Assay for Semi-quantitative, Medium-throughput Enumeration of Mycobacteria

Visualisering af trækul Agar Resazurin Assay til Semi-kvantitativ, Medium-throughput Tælling af mykobakterier

DOI:
10.3791/54690-v

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09:57 min

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December 14, 2016

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, , , , , , , ,
1Departments of Microbiology & Immunology,Weill Cornell Medical College, 2Medicine,Weill Cornell Medical College

Chapters

  • 00:05Title
  • 00:46Preparation of CARA Microplates
  • 02:20Setting up Replicating and Non-replicating MIC90 Assays
  • 04:23Inoculation of CARA Microplates
  • 05:30Developing CARA Microplates and Data Analysis
  • 07:22Results: Enumeration of Mycobacteria via the CARA Assay
  • 09:06Conclusion

Summary

Automatic Translation

Automatic Translation

The charcoal agar resazurin assay (CARA) is a semi-quantitative, medium-throughput method to assess activity of test agents against mycobacteria that are replicating, non-replicating, or both. The CARA permits rapid evaluation of time- and concentration-dependent activity and identifies parameters to pursue by colony forming unit (CFU) assays.

Tags

Keywords: Charcoal Agar Resazurin AssaySemi-quantitativeMedium-throughputMycobacteria EnumerationTuberculosis Drug DiscoveryM. SmegmatisReplicating And Non-replicating Bacteria7H11 AgarOADC Supplement96-well Microplate

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