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September 10, 2016
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Simple methods to detect the selective activation of G proteins by G protein-coupled receptors remain an outstanding challenge in cell signaling. Here, Fӧrster resonance energy transfer (FRET) biosensors have been developed by pairwise tethering a GPCR to G protein peptides to probe conformational changes at controlled concentrations in live cells.
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Cite this Article
Semack, A., Malik, R. U., Sivaramakrishnan, S. G Protein-selective GPCR Conformations Measured Using FRET Sensors in a Live Cell Suspension Fluorometer Assay. J. Vis. Exp. (115), e54696, doi:10.3791/54696 (2016).
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