A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 00:58Cell Culture Preparation and Transfection Conditions
- 02:41Förster Resonance Energy Transfer (FRET) Sensor G Protein-selective G Protein-coupled Receptor (GPCR) Conformation Analysis
- 06:11Results: Optimizing FRET Spectra Generation and Analysis
- 08:01Conclusion
Simple methods to detect the selective activation of G proteins by G protein-coupled receptors remain an outstanding challenge in cell signaling. Here, Fӧrster resonance energy transfer (FRET) biosensors have been developed by pairwise tethering a GPCR to G protein peptides to probe conformational changes at controlled concentrations in live cells.
