G Protein-selective GPCR Conformations Measured Using FRET Sensors in a Live Cell Suspension Fluorometer Assay

Ansley Semack; Rabia U. Malik; Sivaraj Sivaramakrishnan

doi:10.3791/54696

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Conformaciones GPCR Proteína G-selectivos mide usando sensores FRET en un fluorómetro Ensayo de suspensión de células vivas

G Protein-selective GPCR Conformations Measured Using FRET Sensors in a Live Cell Suspension Fluorometer Assay

JoVE Journal
Biology

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JoVE Journal Biology

G Protein-selective GPCR Conformations Measured Using FRET Sensors in a Live Cell Suspension Fluorometer Assay

Conformaciones GPCR Proteína G-selectivos mide usando sensores FRET en un fluorómetro Ensayo de suspensión de células vivas

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9,550 Views

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09:12 min

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September 10, 2016

DOI:

10.3791/54696-v

DOI:

10.3791/54696-v

•

09:12 min

September 10, 2016

•

8 Views

Ansley Semack¹, Rabia U. Malik², Sivaraj Sivaramakrishnan¹

¹Genetics, Cell Biology, and Development,University of Minnesota, ²Department of Cell and Developmental Biology,University of Michigan

Transcript

Summary

Simple methods to detect the selective activation of G proteins by G protein-coupled receptors remain an outstanding challenge in cell signaling. Here, Fӧrster resonance energy transfer (FRET) biosensors have been developed by pairwise tethering a GPCR to G protein peptides to probe conformational changes at controlled concentrations in live cells.