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Analyse af Protein Import til Chloroplaster Isoleret fra Stressede planter
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Analysis of Protein Import into Chloroplasts Isolated from Stressed Plants
DOI:

10:18 min

November 01, 2016

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Chapters

  • 00:05Title
  • 00:46Growth of Arabidopsis Plants and the Stress Treatment
  • 01:34Chloroplast Isolation
  • 05:06Analysis of the Yield and the Intactness of Chloroplasts
  • 06:19Chloroplast Protein Import
  • 08:04Results: Chloroplast Import of the PsaD Protein Is Elevated in the sp1 Mutant under Stress Conditions
  • 09:12Conclusion

Summary

Automatic Translation

Her beskriver vi en ny metode til at studere protein import til isolerede chloroplaster under stress. Fremgangsmåden er enkel og hurtig, og kan anvendes til at undersøge konsekvenserne af forskellige stressbetingelser for chloroplast-protein import, og de tilsvarende reguleringsmekanismer.

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