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Su Kullanımı Endokrin Aktivitesi Tarama Piyasada satılan<em> İn Vitro</em> Transaktivasyon biyolojik tayinler
JoVE Journal
Environment
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JoVE Journal Environment
Screening for Endocrine Activity in Water Using Commercially-available In Vitro Transactivation Bioassays
DOI:

08:00 min

December 04, 2016

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Chapters

  • 00:05Title
  • 01:00Prepare Dilutions of the Assay-specific Reference Chemical and Water Extract
  • 02:42Prepare the Cell Suspension to Conduct the Fret Bioassay
  • 04:05Plate Cells in a 96-well Black Wall Clear-bottom Plate and Add Diluted Water Extract
  • 04:54Measure Cytotoxicity and Endocrine Activity Response
  • 05:51Results
  • 07:22Conclusion

Summary

Automatic Translation

A protocol to screen for endocrine activity in organic extracts of water samples, including treated wastewater effluent and surface (receiving) water, was adapted using commercially available division-arrested ("freeze and thaw") in vitro transactivation bioassays.

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