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Unicellulare espressione genica Utilizzando Multiplex RT-qPCR per caratterizzare eterogeneità delle rare popolazioni linfoidi
JoVE Journal
Genetics
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JoVE Journal Genetics
Single-cell Gene Expression Using Multiplex RT-qPCR to Characterize Heterogeneity of Rare Lymphoid Populations
DOI:

10:23 min

January 19, 2017

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Chapters

  • 00:05Title
  • 00:40Preparing a 96-well Single-cell Sorting Plate
  • 01:55Single-cell Fluorescence-activated Cell Sorting (FACS)
  • 03:02Pre-amplification
  • 03:38Preparing a 96-well Sample Plate
  • 04:23Preparing a 96-well Assay Plate
  • 04:51Preparing and Running the Single-cell Gene-expression Chip
  • 06:52Data Analysis
  • 08:04Results: Single-cell Multiplex RT-qPCR Identifies Cell Heterogeneity of the Liver ILC Compartment
  • 09:33Conclusion

Summary

Automatic Translation

Questo protocollo descrive come valutare l'espressione di una vasta gamma di geni a livello clonale. Single-cell RT-qPCR produce risultati altamente affidabili con una forte sensibilità per le centinaia di campioni e geni.

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