This content is Free Access.
Chapters
- 00:05Title
- 00:43Hydrogel Monomer Infusion and Polymerization
- 01:42Electrophoretic Tissue Clearing
- 02:37Immunolabeling of Dense Tissues
- 05:24Results: PRESTO Immunolabeling of ACT-cleared Dense Tissue
- 06:27Conclusion
ACT-PRESTO (aktiv klarhed teknik-pres relateret effektiv og stabil overførsel af makromolekyler i organer) muliggør hurtig, effektiv, men billig væv clearing og hurtig antistof indtrængen i ryddet væv ved passiv diffusion eller tryk-assisteret levering. Under anvendelse af denne fremgangsmåde, kan en lang række væv blive ryddet, immunolabeled af flere antistoffer, og volumen-afbildes.
Tags
ImmunolabelingVolume ImagingNeuronal ConnectionsMolecular CategorizationStructural ChangeSub-cellular AnalysisHydrogelPolymerizationC-PRESTO (centrifugal-assisted Protein Retention Encoding Through Stochastic Trafficking)S-PRESTO (stochastic Protein Retention Encoding Through Stochastic Trafficking)
