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Chapters
- 00:04Title
- 00:52Tissue Preparation, Delipidation, and Permeabilization
- 03:14Whole Mount Immunostaining
- 04:42Tissue Clearing
- 06:58Results: 3D Imaging of Adipose Tissue
- 08:09Conclusion
Due to the high lipid content, adipose tissue has been challenging to visualize using traditional histological methods. Adipo-Clear is a tissue clearing technique that allows robust labeling and high-resolution volumetric fluorescent imaging of adipose tissue. Here, we describe the methods for sample preparation, pretreatment, staining, clearing, and mounting for imaging.
