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Adipo-Clear: A Tissue Clearing Method for Three-Dimensional Imaging of Adipose Tissue

Article DOI: 10.3791/58271
July 28th, 2018


Summary July 28th, 2018

Due to the high lipid content, adipose tissue has been challenging to visualize using traditional histological methods. Adipo-Clear is a tissue clearing technique that allows robust labeling and high-resolution volumetric fluorescent imaging of adipose tissue. Here, we describe the methods for sample preparation, pretreatment, staining, clearing, and mounting for imaging.


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