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Chapters
- 00:05Title
- 00:49Preparation of Medium and Coating Plate
- 02:09Isolation and Dissociation of the Adult SCZ
- 03:38SCZ-derived aNSC Culture
- 05:18Passaging and Differentiation of SCZ-aNSCs
- 07:22Results: Maintenance of the SCZ-aNSCs as a Monolayer Culture
- 08:17Conclusion
Establishing culture systems for the expansion of adult neural stem cells (aNSCs) allows for the examination and application of aNSCs for therapy. The subcallosal zone (SCZ) has recently been recognized as a novel neuroblast-forming region in adult mice. Here, methods for the isolation, expansion, and differentiation of SCZ-aNSCs are described.
