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支架支持胰岛移植糖尿病小鼠附睾脂肪垫
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Bioengineering
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JoVE Journal Bioengineering
Scaffold-supported Transplantation of Islets in the Epididymal Fat Pad of Diabetic Mice
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支架支持胰岛移植糖尿病小鼠附睾脂肪垫

DOI:
10.3791/54995-v

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11:57 min

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July 23, 2017

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, , , ,
1Department of Biomedical Engineering,Peking University, 2Institute of Molecular Medicine,Peking University

Chapters

  • 00:00Title
  • 00:09Introduction
  • 01:03Cannulation and Perfusion
  • 03:55Purification and Handpicking
  • 06:30Troubleshooting: Common Bile Duct Distended During Perfusion
  • 07:03Troubleshooting: Duodenum Distended During Perfusion
  • 07:37Islet Transplantation at EFP Site
  • 10:23Results
  • 11:29Conclusion

Summary

Automatic Translation

Automatic Translation

该方案证明了鼠胰岛分离和接种到脱细胞支架上。将脚手架支持的胰岛移植到链脲霉素（STZ）诱导的糖尿病小鼠的附睾脂肪垫中。胰岛在移植部位存活并逆转高血糖状况。

Tags

Islet TransplantationScaffold-supportedEpididymal Fat PadDiabetesIslet IsolationCollagenase DigestionHistopaque GradientBeta Cell Replacement Therapy

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