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Bioengineering
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支架支持胰岛移植糖尿病小鼠附睾脂肪垫
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
Scaffold-supported Transplantation of Islets in the Epididymal Fat Pad of Diabetic Mice
Please note that all translations are automatically generated.
Click here for the English version.
支架支持胰岛移植糖尿病小鼠附睾脂肪垫
DOI:
10.3791/54995-v
•
11:57 min
•
July 23, 2017
•
Kai Wang*
1
,
Xi Wang*
1
,
Cheng-sheng Han*
2
,
Liang-yi Chen
,
Ying Luo
1
Department of Biomedical Engineering
,
Peking University
,
2
Institute of Molecular Medicine
,
Peking University
Chapters
00:00
Title
00:09
Introduction
01:03
Cannulation and Perfusion
03:55
Purification and Handpicking
06:30
Troubleshooting: Common Bile Duct Distended During Perfusion
07:03
Troubleshooting: Duodenum Distended During Perfusion
07:37
Islet Transplantation at EFP Site
10:23
Results
11:29
Conclusion
Summary
Automatic Translation
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Automatic Translation
该方案证明了鼠胰岛分离和接种到脱细胞支架上。将脚手架支持的胰岛移植到链脲霉素(STZ)诱导的糖尿病小鼠的附睾脂肪垫中。胰岛在移植部位存活并逆转高血糖状况。
Tags
Islet Transplantation
Scaffold-supported
Epididymal Fat Pad
Diabetes
Islet Isolation
Collagenase Digestion
Histopaque Gradient
Beta Cell Replacement Therapy
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