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dyrking av<em> Caenorhabditis elegans</em> I tre dimensjoner i laboratoriet
JoVE Journal
Biology
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JoVE Journal Biology
Cultivation of Caenorhabditis elegans in Three Dimensions in the Laboratory
DOI:

09:01 min

December 12, 2016

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Chapters

  • 00:05Title
  • 00:52Prepare Solutions and Bacteria Culture for NGT-3D and NGB-3D
  • 02:23Making NGT-3D and NGB-3D
  • 05:18Measure Fitness of Worm Population on NGT-3D
  • 06:47Results: Development, Fertility, and Lifespan of C. elegans Cultivated in NGT-3D Is Comparable with That of the Standard 2D NGM
  • 08:12Conclusion

Summary

Automatic Translation

Vi presenterer en enkel metode for å konstruere 3D-nematode dyrkingssystemer kalt NGT-3D og NGB-3D. Disse kan brukes til å studere nematode fitness og atferd i habitater som er mer lik naturlige Caenorhabditis elegans habitater enn standard 2D-laboratoriet C. elegans kulturplater.

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