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Biochemistry
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实验室规模的生产和治疗性抗体的纯化
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Biochemistry
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JoVE Journal
Biochemistry
Laboratory Scale Production and Purification of a Therapeutic Antibody
Please note that all translations are automatically generated.
Click here for the English version.
实验室规模的生产和治疗性抗体的纯化
DOI:
10.3791/55153-v
•
09:54 min
•
January 24, 2017
•
Zehra Elgundi
,
Vicki Sifniotis
,
Mouhamad Reslan
,
Esteban Cruz
,
Veysel Kayser
1
Faculty of Pharmacy
,
University of Sydney
Chapters
00:05
Title
00:38
Stable Transfection of HEK-293 Cells
03:59
Large Scale Antibody Production from Batch Overgrowth Culture
05:12
Antibody Purification by Affinity Chromatograpy Using an Automated Fast Protein Liquid Chromatography System
07:02
Results: Laboratory-scale Production and Purification of Antibody
08:50
Conclusion
Summary
Automatic Translation
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这个协议描述在哺乳动物表达系统中生产的治疗性抗体的。所描述的方法包括制备载体DNA,稳定转染和人胚肾293细胞系的无血清适应的,设置大规模培养和纯化使用亲和层析组成。
Tags
Antibody Production
Therapeutic Antibody
Serum-free Expression
HEK-293 Cells
Stable Cell Line
Transfection
Polyethylenimine (PEI)
Hygromycin B
Serum-free Adaptation
Purification
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