JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Developmental Biology
/
新生小鼠胰腺胰岛功能的有效隔离
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Developmental Biology
Effective Isolation of Functional Islets from Neonatal Mouse Pancreas
Please note that all translations are automatically generated.
Click here for the English version.
新生小鼠胰腺胰岛功能的有效隔离
DOI:
10.3791/55160-v
•
07:04 min
•
January 06, 2017
•
Chen Huang
,
Guoqiang Gu
1
Department of Cell and Development Biology
,
Vanderbilt University Medical School
Chapters
00:05
Title
00:39
Pancreas Isolation and Digestion
02:14
Lysate Washing and Islet Isolation
04:42
Results: Representative Islet Isolation Analyses
05:39
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
我们在此描述了一个协议,从新生小鼠分离胰岛完好无损。胰腺被部分用胶原酶消化,接着洗涤和手采摘。 20 - 80胰岛集群可以每胰腺从新生小鼠,这是适合于几个胰岛研究而获得。
Tags
Neonatal Mouse Pancreas
Islet Isolation
Collagenase Digestion
HBSS
RPMI 1640
Polysucrose
Sodium Diatrazoate
Insulin Secretion
Gene Expression
Paracell Survival
Functional Maintenance
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
隔离和乳鼠心肌细胞冷冻保存
体外
测定菌落为表征的三效祖细胞从成人胰腺小鼠隔离
小鼠胚胎Hemogenic内皮细胞的分离
从小鼠骨骼肌分离I型和II型周细胞
用高频超声 (30/45 mhz) 系统对胎儿小鼠进行心血管成像
高效生成粘附培养中 Hpsc 的胰腺-二叶多布蛋白 1
+
后后福格内胰腺祖细胞
特定神经元种群与圆虫
卡埃纳哈布迪炎的
分离
小鼠骨骼肌肉外泄原发性肌基器的分离和分化
从小鼠和人类牙齿中快速分离单个细胞
从小鼠面部过程和培养的腭间充质细胞中分离全细胞蛋白质裂解物以进行磷酸化蛋白分析
Read Article