A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 00:51Conjugation of PEG-Chol to DiO-LCNP via EDC Coupling and Characterization of the Product
- 02:33Preparation of Cell Culture Dishes for Delivery Experiments and Imaging
- 04:07Cellular Delivery of DiO and DiO-LCNPs and Imaging of Fixed Cells
- 06:11Cellular Delivery DiO-LCNPs and DiI FRET Imaging in Live Cells
- 07:37Results: Analysis of DiO-LCNP-PEG-Chol Cellular Uptake to the Plasma Membrane and Quantification of Cytotoxicity
- 08:57Conclusion
