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Journal
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Bioengineering
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疏水货物封装有针对性的质膜交付在液晶纳米颗粒载体
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
Targeted Plasma Membrane Delivery of a Hydrophobic Cargo Encapsulated in a Liquid Crystal Nanoparticle Carrier
Please note that all translations are automatically generated.
Click here for the English version.
疏水货物封装有针对性的质膜交付在液晶纳米颗粒载体
DOI:
10.3791/55181-v
•
10:16 min
•
February 08, 2017
•
Okhil K. Nag
,
Jawad Naciri
,
Eunkeu Oh
3
,
Christopher M. Spillmann
,
James B. Delehanty
1
Center for Biomolecular Science and Engineering
,
Naval Research Laboratory
,
2
Optical Sciences Division
,
Naval Research Laboratory
,
3
Sotera Defense Solutions
Chapters
00:05
Title
00:51
Conjugation of PEG-Chol to DiO-LCNP via EDC Coupling and Characterization of the Product
02:33
Preparation of Cell Culture Dishes for Delivery Experiments and Imaging
04:07
Cellular Delivery of DiO and DiO-LCNPs and Imaging of Fixed Cells
06:11
Cellular Delivery DiO-LCNPs and DiI FRET Imaging in Live Cells
07:37
Results: Analysis of DiO-LCNP-PEG-Chol Cellular Uptake to the Plasma Membrane and Quantification of Cytotoxicity
08:57
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
一种液晶纳米颗粒(LCNP)纳米载体被利用作为用于受控递送疏水货物的活细胞的质膜的车辆。
Tags
Liquid Crystal Nanoparticle
Hydrophobic Cargo
Plasma Membrane Delivery
PEG Cholesterol Conjugation
Gel Electrophoresis
HEK 293 T17 Cell Culture
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