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Gezielte Plasma Membrane Lieferung eines Hydrophobic Transport Encapsulated in einem Liquid Crystal Nanoparticle Träger
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Targeted Plasma Membrane Delivery of a Hydrophobic Cargo Encapsulated in a Liquid Crystal Nanoparticle Carrier
DOI:

10:16 min

February 08, 2017

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Chapters

  • 00:05Title
  • 00:51Conjugation of PEG-Chol to DiO-LCNP via EDC Coupling and Characterization of the Product
  • 02:33Preparation of Cell Culture Dishes for Delivery Experiments and Imaging
  • 04:07Cellular Delivery of DiO and DiO-LCNPs and Imaging of Fixed Cells
  • 06:11Cellular Delivery DiO-LCNPs and DiI FRET Imaging in Live Cells
  • 07:37Results: Analysis of DiO-LCNP-PEG-Chol Cellular Uptake to the Plasma Membrane and Quantification of Cytotoxicity
  • 08:57Conclusion

Summary

Automatic Translation

Eine Flüssigkristall-Nanopartikel (LCNP) Nanotransporter wird als Träger für die kontrollierte Abgabe eines hydrophoben Ladung an die Plasmamembran lebender Zellen ausgenutzt.

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