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Chapters
- 00:05Title
- 00:51Preparation
- 04:12Fly-dusting and Grooming
- 06:12Preparation of Samples and Absorbance Analysis
- 08:08Results: DopR Plays Significant Role in Grooming Behavior
- 08:52Conclusion
This protocol describes a scalable individual grooming assay technique in Drosophila that yields robust, quantitative data to measure grooming behavior. The method is based on comparing the difference in dye accumulation on the bodies of un-groomed versus groomed animals over a set period of time.
Tags
Keywords:
DrosophilaGrooming BehaviorQuantificationBrilliant Yellow DyeAspiratorGrooming ChamberMicro-pipette TipTygon TubingEthanolNeurobiologyBehavioral GeneticsFine Locomotive SkillsMolecular RegulatorsCircuitsRapidRobustEasy-to-performStudentCulture VialMeshVacuum PressureWeighing PaperMicro-centrifuge TubeMicro-pipette TipNegative Control
