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Journal / Genetics / Kortlægning af RNA-RNA-interaktioner globalt ved…
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JoVE Journal Genetics
Kortlægning af RNA-RNA-interaktioner globalt ved anvendelse af biotinyleret psoralen
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Kortlægning af RNA-RNA-interaktioner globalt ved anvendelse af biotinyleret psoralen

Article DOI: 10.3791/55255-v • 11:32 min • May 24th, 2017
May 24th, 2017 •
1, 2, 2, 1
1Stem Cell and Regenerative Biology, Genome Institute of Singapore, A*STAR, 2Computational and Systems Biology, Genome Institute of Singapore, A*STAR

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Her beskriver vi metoden for S- ækvurdering af P soralen tværbundet, L igateret og S valgte H ybrids (SPLASH), som muliggør genomgående kortlægning af intramolekylære og intermolekylære RNA-RNA-interaktioner in vivo . SPLASH kan anvendes til at studere RNA-interaktomer af organismer, herunder gær, bakterier og mennesker.

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Genetik RNA genomics interactome sequencing struktur human
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