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Chapters
- 00:05Title
- 01:12Treatment of HeLa Cells with Biotinylated Psoralen RNA Extraction, and Fragmentation
- 02:25RNA Size Selection and Elution
- 04:41Enrichment of RNA Crosslinking Regions
- 05:57Proximity Ligation
- 07:34Reverse Crosslinking of Biotinylated Psoralen, and Amplification of cDNA
- 09:13The SPLASH Workflow and Representative Results
- 10:50Conclusion
Aquí, se detalla el método de S de equación de P soralen reticulado, L igated, y S elegido H ybrids (SPLASH), que permite el genoma de la cartografía de todo el intramolecular y intermolecular RNA-ARN interacciones in vivo . SPLASH puede ser aplicado para estudiar interactones de ARN de organismos incluyendo levaduras, bacterias y seres humanos.
