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Biochemistry
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差示扫描量热 - 用于评估蛋白质抗原的热稳定性和构象的方法
JoVE Journal
Biochemistry
This content is Free Access.
JoVE Journal
Biochemistry
Differential Scanning Calorimetry — A Method for Assessing the Thermal Stability and Conformation of Protein Antigen
Please note that all translations are automatically generated.
Click here for the English version.
差示扫描量热 - 用于评估蛋白质抗原的热稳定性和构象的方法
DOI:
10.3791/55262-v
•
08:13 min
•
March 04, 2017
•
Ibrahim B. Durowoju
,
Kamaljit S. Bhandal
,
Jian Hu
,
Bruce Carpick
,
Marina Kirkitadze
1
Analytical Research & Development
,
Sanofi Pasteur Limited
Chapters
00:05
Title
01:11
Instrument Start-up
02:08
Sample Preparation
03:46
Experimental Parameter Setup
05:46
Results: Thermal Transitions of Vaccine Antigens
06:59
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
差示扫描量热法测量,以变性的蛋白质所需要的热转变温度(S)和总的热能量。得到的结果用于评估疫苗制剂的蛋白质抗原的热稳定性。
Tags
Differential Scanning Calorimetry
Thermal Stability
Protein Antigen
Structural Conformation
Molar Heat Capacity
Thermal Transition Temperature
Enthalpy Of Unfolding
Lot-to-lot Consistency
Biologics
Protein Concentration
Lowry Method
Buffer Equilibration
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