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基因组编辑和hPSCs定向分化为胰腺癌发展讯问谱系行列式
JoVE Journal
Genetics
This content is Free Access.
JoVE Journal
Genetics
Genome Editing and Directed Differentiation of hPSCs for Interrogating Lineage Determinants in Human Pancreatic Development
Please note that all translations are automatically generated.
Click here for the English version.
基因组编辑和hPSCs定向分化为胰腺癌发展讯问谱系行列式
DOI:
10.3791/55267-v
•
09:37 min
•
March 05, 2017
•
Zhong-Dong Shi*
1
,
Chew-Li Soh*
1
,
Zengrong Zhu*
1
,
Danwei Huangfu
1
Developmental Biology Program
,
Sloan Kettering Institute
Chapters
00:05
Title
00:52
Generation of iCas9 hPSC Lines
04:15
gRNA Transfection of iCas9 hPSCs
06:00
In Vitro
hPSC Differentiation into Glucose-responsive Pancreatic β Cells
07:48
Results: Efficient Genetic Modification in hPSCs Using the iCRISPR Platform
08:50
Conclusion
Summary
Automatic Translation
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Automatic Translation
协议来生成使用iCRISPR平台HPSC突变系和分化hPSCs成葡萄糖反应β样细胞中有描述。结合基因组编辑技术,HPSC-定向分化为血统决定因素在人的发展和疾病发展中的作用的系统分析了强大的平台。
Tags
Genome Editing
Directed Differentiation
HPSCs
Pancreatic Development
ICRISPR
Vitronectin
ROCK Inhibitor
Electroporation
Cell Culture
Cell Suspension
Cell Count
Plasmids
Gene Modification
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