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Journal
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Biochemistry
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使用SPHIRE的电子冷冻显微镜图像的高分辨率单粒子分析
JoVE Journal
Biochemistry
This content is Free Access.
JoVE Journal
Biochemistry
High-resolution Single Particle Analysis from Electron Cryo-microscopy Images Using SPHIRE
Please note that all translations are automatically generated.
Click here for the English version.
使用SPHIRE的电子冷冻显微镜图像的高分辨率单粒子分析
DOI:
10.3791/55448-v
•
13:28 min
•
May 16, 2017
•
Toshio Moriya
,
Michael Saur
,
Markus Stabrin
,
Felipe Merino
,
Horatiu Voicu
,
Zhong Huang
,
Pawel A. Penczek
,
Stefan Raunser
,
Christos Gatsogiannis
1
Department of Structural Biochemistry
,
Max Planck Institute of Molecular Physiology
,
2
Department of Biochemistry and Molecular Biology
,
The University of Texas Medical School at Houston
Chapters
00:05
Title
01:16
Micrograph Alignment and Particle Picking
03:48
2D Classification and Initial 3D Modeling
05:51
3D Model-refinement
07:53
3D Sorting and Local Resolution Estimation
10:46
Results: High-resolution 3D Modeling of TcdA1
12:19
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本文介绍了使用软件套件SPHIRE处理低温EM图像的协议。目前的协议可以应用于几乎所有瞄准近原子分辨率的单粒子EM项目。
Tags
Single Particle Analysis
Electron Cryo-microscopy
SPHIRE
Macromolecular Complexes
High-resolution Cryo-EM
Particle Box Size
CTF Estimation
Motion Correction
Dose-weighted
Dose-unweighted
Particle Picking
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