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JoVE Journal
Behavior
Battery of Behavioral Tests Assessing General Locomotion, Muscular Strength, and Coordination in ...
Battery of Behavioral Tests Assessing General Locomotion, Muscular Strength, and Coordination in ...
JoVE Journal
Behavior
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JoVE Journal Behavior
Battery of Behavioral Tests Assessing General Locomotion, Muscular Strength, and Coordination in Mice

Battery of Behavioral Tests Assessing General Locomotion, Muscular Strength, and Coordination in Mice

Full Text
14,600 Views
06:54 min
January 23, 2018

DOI: 10.3791/55491-v

Karlaina JL Osmon1, Meera Vyas1, Evan Woodley2, Patrick Thompson2, Jagdeep S Walia1,2,3

1Centre for Neuroscience Studies,Queen's University, 2Department of Biomedical and Molecular Sciences,Queen's University, 3Medical Genetics/ Department of Pediatrics, Kingston General Hospital, Centre for Neuroscience Studies, and Department of Biomedical and Molecular Sciences,Queen's University

Behavioral testing is used in pre-clinical trials to assess the phenotypic effects of a disease or treatment on an animal's wellbeing. In order to globally assess motor functioning, we selected tests for general locomotion, muscular strength, and coordination: the open field test, the mesh test, and the rotarod test, respectively.

The overall goal of this battery of behavioral testing is to observe the general locomotion, muscular strength, and coordination in mice. These methods can help answer key questions about the implementation of different treatments in multiple preclinical trials, such as the benefit of treatment outcomes on motility in mice. The main advantage of this technique is that multiple facets and dimensions of movement are measured and assessed by this battery of behavioral testing.

Demonstrating the procedure will be Meera, a master student in our lab. Begin by turning on the open field test program, and connecting the computer to the camera or open field box. Start the selective SmartWare software in the Open Field icon.

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Behavioral TestingLocomotionMuscular StrengthCoordinationMiceOpen Field TestMesh TestRotarod TestPreclinical TrialsMovementMotilitySmartWare SoftwareDigital Analog ConverterCalibrationData AnalysisAccelerated Protocol

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