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Chapters
- 00:05Title
- 00:50Setup Prior to Recordings
- 01:35Seeding and Transfection of Cells
- 02:50Selectivity Measurements
- 06:48Results: Chloride Selectivity of PsACR1
- 07:51Conclusion
This article describes how the ion selectivity of channelrhodopsin is determined with electrophysiological whole-cell patch-clamp recordings using HEK293 cells. Here, the experimental procedure for investigating chloride selectivity of an anion-selective channelrhodopsin is demonstrated. However, the procedure is transferable to other channelrhodopsins of distinct selectivity.
