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CAPRRESI: Chimera Assembly by Plasmid Recovery and Restriction Enzyme Site Insertion
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CAPRRESI: Chimera Assembly by Plasmid Recovery and Restriction Enzyme Site Insertion

CAPRRESI: Chimera Assembly by Plasmid Recovery and Restriction Enzyme Site Insertion

DOI:
10.3791/55526-v

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07:37 min

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June 25, 2017

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, ,
1Centro de Ciencias Genómicas,Universidad Nacional Autónoma de México, 2Oncomedic Incorporation, Ciudad de México, 3Laboratorio Internacional de Investigación sobre el Genoma Humano,Universidad Nacional Autónoma de México

Chapters

  • 00:05Title
  • 01:22CAPRRESI Oligonucleotide Design
  • 03:29Plasmid Disruption by PCR Followed by Restriction Enzyme Digestion
  • 05:15Plasmid Recovery Using DNA Ligation and Bacterial Transformation
  • 06:43Results: Example Sequence of Assembled Gene
  • 07:18Conclusion

Summary

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Here, we present chimera assembly by plasmid recovery and restriction enzyme site insertion (CAPRRESI), a protocol based on the insertion of restriction enzyme sites into synonym DNA sequences and functional plasmid recovery. This protocol is a fast and low-cost method for fusing protein-coding genes.

Tags

CAPRRESIChimera AssemblyPlasmid RecoveryRestriction Enzyme Site InsertionGene FusionMolecular BiologyRpoDSigAAmpicillin ResistancePCRPerl ScriptsAmino Acid Sequence AlignmentSynonym DNARestriction Enzyme Sites

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