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Piccolo RNA trasfezione in cellule primarie Th17 Human Next Generation elettroporazione
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Immunology and Infection
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JoVE Journal Immunology and Infection
Small RNA Transfection in Primary Human Th17 Cells by Next Generation Electroporation
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Piccolo RNA trasfezione in cellule primarie Th17 Human Next Generation elettroporazione

DOI:
10.3791/55546-v

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10:15 min

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April 13, 2017

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1Department of Microbiology & Immunology, Sandler Asthma Basic Research Center,University of California, San Francisco

Chapters

  • 00:05Title
  • 00:48T Cell Culture Preparation and CD4+ T Cell Isolation
  • 03:38Th17 Polarization
  • 04:41Electroporation of In Vitro Polarized Human Th17 Cells
  • 08:03Results: Robust In Vitro Differentiated Human Th17 Cell Cultures
  • 09:48Conclusion

Summary

Automatic Translation

Automatic Translation

Next generation electroporation is an efficient method for transfecting human Th17 cells with small RNAs to alter gene expression and cell behavior.

Tags

Keywords: Small RNA TransfectionPrimary Human Th17 CellsNext Generation ElectroporationT Cell BiologyGene RegulationT Cell DifferentiationImmune FunctionHuman Th17 CellsLymphocytesCord-blood Mononuclear CellsCD4 Positive T Cell IsolationNegative SelectionMagnetic BeadsCell CultureCell IsolationCell WashingCell IncubationCell Resuspension

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