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Isolering af murine fedtvæv-afledte mikrovaskulær Fragmenter som Vaskularisering Enheder til Tissue Engineering
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Bioengineering
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JoVE Journal Bioengineering
Isolation of Murine Adipose Tissue-derived Microvascular Fragments as Vascularization Units for Tissue Engineering

Isolering af murine fedtvæv-afledte mikrovaskulær Fragmenter som Vaskularisering Enheder til Tissue Engineering

DOI:
10.3791/55721-v

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07:09 min

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April 30, 2017

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, , , ,
1Institute for Clinical and Experimental Surgery,Saarland University, 2Division of Plastic Surgery and Hand Surgery,University Hospital Zurich, University of Zurich

Chapters

  • 00:05Title
  • 00:54Epididymal Fat Pad Harvesting
  • 02:07Adipose Tissue-derived Microvascular Fragment (ad-MVF) Isolation
  • 05:05Representative Results: ad-MVF Characterization
  • 06:38Conclusion

Summary

Automatic Translation

Automatic Translation

Vi præsenterer en protokol til isolering fedtvævslidelser-afledte mikrovaskulære fragmenter, der repræsenterer lovende vaskularisering enheder. De kan hurtigt isoleres, ikke kræver in vitro behandling og således kan anvendes til et-trins prevascularization i forskellige områder af vævsmanipulering.

Tags

Microvascular FragmentsVascularizationIn VivoAngiogenesisMurineC57 Black SixLaparotomyEpididymal Fat PadsDMEMCollagenaseDigestionCell Culture IncubatorMicroscopy

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