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September 15, 2017
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The overall goal of this screening assay is to characterize novel endothelial regulators involved in the inflammatory response of interest for designing new therapeutic strategies against inflammatory disorders.This method can help answer key questions in the pathology field such as defining novel endothelial-targeting drugs able to attenuate inflammatory-related diseases.The main advantage of this endothelial protocol is that describes assay technology that establishes the basis for exploring novel mechanisms involved in the regulation of the inflammatory response.Demonstrating this methodology will be Maria Angeles Higueras, a post-doc from our lab and Sandra Herranz, a technician from our lab.To begin, culture MLEC-04 cells in complete DMEM F12 medium supplemented with 10%FBS and Pen-Strep.Incubate the cells in a biological incubator at 37
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血管内皮細胞は、しっかりと白血球動員を制御します。不十分な白血球の血管外漏出は、炎症性疾患に貢献します。したがって、内皮活性化の新しい規制要素の検索は、炎症性疾患の改善治療を設計する必要です。ここでは、白血球は炎症時に人身売買を変更できる新しい内皮調節を特徴付けるための包括的な方法論について述べる。
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Higueras, M. Á., Jiménez-García, L., Herranz, S., Hortelano, S., Luque, A. Screening Assays to Characterize Novel Endothelial Regulators Involved in the Inflammatory Response. J. Vis. Exp. (127), e55824, doi:10.3791/55824 (2017).
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