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Screening Assays for at karakterisere romanen endotel regulerende myndigheder involveret i inflammatorisk reaktion
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Chapters
- 00:05Title
- 00:58Endothelial Cell Culture and Tissue Culture Conditions
- 02:42Evaluate Endothelial Factor Released on Leukocyte Activation by Adhesion Assays
- 04:12Leukocyte Adhesion Assay
- 07:42Test Endothelial Activation by Leukocyte Endothelium Co-adhesion Assay
- 09:59Results: Activation Parameters of LPS-stimulated Endothelial Cells and Influence on Leukocyte Behavior
- 11:52Conclusion
Vaskulære endotel styrer stramt leukocyt rekruttering. Utilstrækkelig leukocyt ekstravasation bidrager til menneskers inflammatoriske sygdomme. Det er derfor nødvendigt at designe bedre behandlinger til inflammatoriske lidelser søger roman regulerende elementer i endothelial aktivisering. Her beskriver vi en omfattende metode til at karakterisere romanen endotel regulatorer, der kan ændre leukocyt handel under betændelse.
