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September 15, 2017
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The overall goal of this screening assay is to characterize novel endothelial regulators involved in the inflammatory response of interest for designing new therapeutic strategies against inflammatory disorders.This method can help answer key questions in the pathology field such as defining novel endothelial-targeting drugs able to attenuate inflammatory-related diseases.The main advantage of this endothelial protocol is that describes assay technology that establishes the basis for exploring novel mechanisms involved in the regulation of the inflammatory response.Demonstrating this methodology will be Maria Angeles Higueras, a post-doc from our lab and Sandra Herranz, a technician from our lab.To begin, culture MLEC-04 cells in complete DMEM F12 medium supplemented with 10%FBS and Pen-Strep.Incubate the cells in a biological incubator at 37
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Vaskulära endotel styr tätt leukocyt rekrytering. Otillräcklig leukocyt extravasering bidrar till inflammatoriska sjukdomar. Söka efter romanen reglerande element av endothelial aktivering därför nödvändigt att utforma förbättrade terapier för inflammatoriska sjukdomar. Här beskriver vi en omfattande metod för att karakterisera roman endothelial regulatorer som kan ändra leukocyt människohandel vid inflammation.
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Higueras, M. Á., Jiménez-García, L., Herranz, S., Hortelano, S., Luque, A. Screening Assays to Characterize Novel Endothelial Regulators Involved in the Inflammatory Response. J. Vis. Exp. (127), e55824, doi:10.3791/55824 (2017).
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