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胚胎运动神经元轴突投影模式的可视化<em>果蝇</em

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Visualization of the Axonal Projection Pattern of Embryonic Motor Neurons in Drosophila

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胚胎运动神经元轴突投影模式的可视化<em>果蝇</em

DOI:

10.3791/55830-v

•

11:56 min

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June 16, 2017

•

Sangyun Jeong

¹Department of Molecular Biology,Chonbuk National University

Chapters

00:05Title
00:48Preparation of Late-stage Embryos for Immunostaining
04:19LacZ Staining and Immunostaining
07:20Preparing Embryos to Image the ISN Nerves in Abdominal Segments 7 to A9
10:03Results: Intersegmental Nerve Bundle (ISNb) Innervation at A3 and A4
11:21Conclusion

Summary

Automatic Translation

这项工作详细描述了一个标准的免疫组织化学方法，可视化晚期16 号果蝇黑色素瘤胚胎的运动神经元投影。用FasII抗体染色的固定胚胎的切片制备提供了一个强大的工具，用于表征神经发育期间运动轴索寻路和靶标识别所需的基因。

Tags

Axonal Projection Pattern Motor Neurons Drosophila Neural Development Motor Axon Guidance Neural Subcircuit Formation Visualization Embryos PBT Solution Dechorionation Devitellinization LacZ Staining X-gal Staining Solution

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