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Visualisering af Axonal Projeksionsmønsteret af Embryoniske Motor Neuroner i<em> Drosophila</em
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Visualization of the Axonal Projection Pattern of Embryonic Motor Neurons in Drosophila
DOI:

11:56 min

June 16, 2017

Chapters

  • 00:05Title
  • 00:48Preparation of Late-stage Embryos for Immunostaining
  • 04:19LacZ Staining and Immunostaining
  • 07:20Preparing Embryos to Image the ISN Nerves in Abdominal Segments 7 to A9
  • 10:03Results: Intersegmental Nerve Bundle (ISNb) Innervation at A3 and A4
  • 11:21Conclusion

Summary

Automatic Translation

Dette arbejde beskriver en standard immunohistokemi metode til at visualisere motor neuron projiceringer af sen fase-16 Drosophila melanogaster embryoner . Det fileterede præparat af faste embryoner farvet med FasII-antistof giver et kraftfuldt værktøj til at karakterisere de gener, der kræves til motorisk axon pathfinding og målgenkendelse under neurale udvikling.

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