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体外
生物荧光法对乳腺上皮细胞昼夜节律的表征
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Genetics
In Vitro
Bioluminescence Assay to Characterize Circadian Rhythm in Mammary Epithelial Cells
Please note that all translations are automatically generated.
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体外
生物荧光法对乳腺上皮细胞昼夜节律的表征
DOI:
10.3791/55832-v
•
11:56 min
•
September 28, 2017
•
Mingzhu Fang
,
Hwan-Goo Kang
,
Youngil Park
,
Brian Estrella
,
Helmut Zarbl
1
Department of Environmental and Occupational Health, School of Public Health, NIEHS Center for Environmental Exposures and Disease, Environmental and Occupational Health Sciences Institute
,
Rutgers University
,
2
Veterinary Drugs & Biologics Division
,
Animal and Plant Quarantine Agency
Chapters
00:05
Title
00:53
Concstruction of
PER2
Promoter Driven Destabilized Luciferase Reporter Vector
03:06
Transforming Ligated Vector pGL[hPer2P/Luc2P/Neo] to
E. coli
05:28
Transient Transfection of Cells with the Circadian Vector
07:46
Establishment of the
In Vitro
Bioluminescence Assay in Transiently Transfected MCF10A Cells
08:52
Results: Effects of Chemicls on the Cellular Biological Clock
10:16
Conclusion
Summary
Automatic Translation
English (Original)
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français (French)
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Automatic Translation
一个
体外
生物荧光法测定乳腺上皮细胞的细胞昼夜节律。该方法利用哺乳动物细胞报告质粒表达不稳定的荧光在
周期 2
基因启动子的控制下。它可以适应其他细胞类型, 以评估器官特定的影响昼夜节律。
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