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一种优化的血凝抑制 (HI) 测定流感特异抗体滴
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JoVE Journal Medicine
An Optimized Hemagglutination Inhibition (HI) Assay to Quantify Influenza-specific Antibody Titers
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一种优化的血凝抑制 (HI) 测定流感特异抗体滴

DOI:
10.3791/55833-v

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06:34 min

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December 01, 2017

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, , , , , ,
1Applied Microbiology Research, Department of Biomedicine,University of Basel, 2Department of Biosystems Science and Engineering,ETH Zurich, 3Swiss Institute of Bioinformatics, 4Clinical Microbiology,University Hospital Basel

Chapters

  • 00:05Title
  • 00:54Hemagglutination Inhibition (HI) Assay
  • 03:28Results: Representative Vacination-induced Antibody Responses Against Influenza
  • 05:11Conclusion

Summary

Automatic Translation

Automatic Translation

本协议描述了如何进行血凝抑制试验, 以量化流感疫苗接受者血清样本中的流感特异性抗体滴。第一种方法是通过血凝法确定最佳病毒抗原浓度。第二种方法通过血凝抑制来量化流感特异抗体滴。

Tags

Hemagglutination Inhibition AssayInfluenza-specific Antibody TitersVaccine-mediated ImmunityNeutralizing AntibodiesMouse SerumCulture SupernatantsAntigen SolutionRDE Treated Serum SamplesAntiserumSerial DilutionsRed Blood Cell SolutionIncubation TimeHemagglutination

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