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Chapters
- 00:05Title
- 01:08Preparation of the Latex Cap and Intraventricular Balloon
- 03:06Isolation and Cannulation of the Heart
- 04:34Reversed-heart Model
- 06:24Results: Protein and Purine Analysis of Cardiac Transudate
- 07:33Conclusion
Denne protokol beskriver en metode til at indsamle hjerteinterstitielvæske fra det isolerede, perfuserede rottehjerte. For fysisk at adskille interstitialtransudat fra koronar venøs effluentperfusus, er Langendorff-perfusionshjertet omvendt, og transudatet (interstitialvæsken) dannet på hjerteoverfladen opsamles under anvendelse af en blød latexhætte.
Tags
Inverted Heart ModelInterstitial Transudate CollectionIsolated Rat HeartParacrine FactorsInterstitial Fluid SpaceCardiac CellsInflammationRegenerationInfarcted MyocardiumLatex Heart CoverMoldLiquid LatexSilicon TubingCardiac TransudateVentricular BalloonPressure TransducerLangendorff Perfusion System
