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Developmental Biology
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从小鼠骨骼肌分离I型和II型周细胞
JoVE Journal
Developmental Biology
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JoVE Journal
Developmental Biology
Isolation of Type I and Type II Pericytes from Mouse Skeletal Muscles
Please note that all translations are automatically generated.
Click here for the English version.
从小鼠骨骼肌分离I型和II型周细胞
DOI:
10.3791/55904-v
•
10:07 min
•
May 26, 2017
•
Abhijit Nirwane
,
Jyoti Gautam
,
Yao Yao
1
College of Pharmacy
,
University of Minnesota
Chapters
00:05
Title
00:47
Muscle Dissection and Single-cell Isolation
03:02
Cell Sorting
07:03
Post-sorting Analyses
08:22
Results: Distinct Characteristics of Type I and Type II Pericytes
09:35
Conclusion
Summary
Automatic Translation
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Automatic Translation
这项工作描述了基于FACS的协议,允许从骨骼肌容易和同时地分离I型和II型周细胞。
Tags
Pericyte Isolation
Type I Pericyte
Type II Pericyte
Skeletal Muscle
FACS
Nestin-GFP
Collagenase
Trypsin-EDTA
Red Blood Cell Lysis
Cell Sorting
Cell Suspension
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